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Establishment of iPS Cells

Method for the Episomal Plasmide Vector

Here, we introduce the method of establishing iPS cells from a peripheral blood monocyte by introducing the initialization factor with the episomal plasmid. This is the low risk method for chromosome integration of exogenously introduced genes.. The feeder-free method is employed with the StemFit Basic medium and iMatrix-511.

(i) Preparation of the plate

(ii) Establishment by the episomal plasmid vector

Equipment/Reagent to be Used

Point of Operation

[Gene transfer]
Take special care not to introduce air bubbles when transferring to the cuvette for electroporation.

[After gene transfer]
Immediately after introducing the gene to the cells by electroporation, suspend the cells in the medium previously warmed to 37°C in 5% CO2 incubator and inoculate onto a plate.

[After culture initiation]
To prevent flotation of the adherent monocyte, the StemFit® medium will be added carefully along the wall of the well.
* This content is provided as reference information for your own experiments and research. This information is not a guarantee of results.

Establishment with Virus Vector

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